Fluorescence techniques

optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation

energy transfer mechanism and microscopy technique

Fluorescence imaging technique

optical fluorescence microscopy

experimental technique in cell biology

microscope to observe a very shallow depth in a cell

type of statistical analysis

term

Fluorescence microscopy technique

effect

biochemical method

The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene. GFP was isolated from the jelly fish Aequorea victoria. Because it shares a bidirectional promoter with a gene for metabolizing arabinose, the GFP gene is expressed in the presence of arabinose, which makes the transgenic organism express its fluorescence under UV light. GFP can be induced in bacteria containing the pGLO plasmid by growing them on