Monotypic fly genera

Hesperinus is a genus of flies and the sole genus in the relict family Hesperinidae belonging to the nematoceran infraorder Bibionomorpha. There are about 8 known species, nearly all from the Palaearctic region with one each from the Nearctic and Neotropical regions. Three fossil species from Eocene Baltic amber have been described. These flies have long 12-segmented antennae, legs and abdomen and males have well-developed wings while females have a short one. Little is known, but most species have been collected near streams in woodlands.

Axymyia is a genus of nematoceran flies in the family Axymyiidae. It contains only one described species, Axymyia furcata, from eastern North America. A second species, Axymyia japonica , is sometimes listed, but this species is now generally placed in the related genus Protaxymyia.

Usambaromyia nigrala is a species of African non-biting midges, and is the only species in the subfamily Usambaromyiinae.

Aymaramyia is a genus of crane fly, Tipuloidea, in the family Limoniidae. There is only one known species.

Strobliella is a genus of midges in the family Cecidomyiidae. The one described species - Strobliella intermedia - is found in the Holarctic region. The genus was established in 1898 by Jean-Jacques Kieffer.

Corinthomyia is a genus of midges in the family Cecidomyiidae. The one described species - Corinthomyia brevicornis - is found in the Holarctic region. The genus was established in 1911 by American entomologist Ephraim Porter Felt.

Neocatocha is a genus of wood midges, insects in the family Cecidomyiidae. The one described species - Neocatocha marilandica - has a holarctic distribution. The genus was established by American entomologist Ephraim Porter Felt in 1912.

Araucoxenia is a genus of crane fly in the family Limoniidae. There is only one known species.

Skuhraviana is a genus of midges in the family Cecidomyiidae. The one described species - Skuhraviana triangulifera - is found in the Holarctic region. The genus was established in 1963 by Soviet entomologist Boris Mamaev.