Category
page 1Peptide sequences
protein tag
artificial peptide attached to protein for marking purpose
amino acid sequence
any continuous part of a peptide/protein
degron
thumb|300x300px|Shown here in green is a portion of IκBα, an inhibitor of NF-κB and regulator of the immune system. The red region highlights the sixth [[ankyrin repeat domain, which contains a ubiquitin-independent degron.]]
A degron is a portion of a protein that is important in regulation of protein degradation rates. Known degrons include short amino acid sequences, structural motifs and exposed amino acids (often lysine or arginine) located anywhere in the protein. In fact, some proteins can even contain multiple degrons. Degrons are present in a variety of organisms, from the N-degrons (
FLAG-tag
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expresse
Sequon
A sequon is a sequence of consecutive amino acids in a protein that can serve as the attachment site to a polysaccharide, frequently an N-linked-Glycan.
The polysaccharide is linked to the protein via the nitrogen atom in the side chain of asparagine (Asn). The sequon for N-glycosylation is either Asn-X-Ser or Asn-X-Thr, where X is any amino acid except proline, Ser denoting serine and Thr threonine.
Occasionally, other amino acids can take the place of Ser and Thr, such as in the leukocyte surface protein (CD69), where the amino acid sequence Asn-X-Cys is an acceptable sequon for the addition
KDEL
Target peptide sequence