Polymerase chain reaction

in vitro method for producing large amounts of specific DNA or RNA fragments from small amounts of short oligonucleotide primers

short strand of RNA or DNA that serves as a starting point for DNA synthesis

method for separation and analysis of macromolecules

species of bacterium

laboratory technique of molecular biology based on the polymerase chain reaction (PCR)

class of enzymes

method of separating chemical or biological samples

method in molecular biology; variant of polymerase chain reaction (PCR), is a technique commonly used in molecular biology to detect RNA expression

isolation of DNA

physicoanalytical technique, electrophoresis in which agar or agarose gel is used as the diffusion medium

laboratory equipment used to change temperature in a programmed cycle

highly sensitive tool used in molecular biology to detect DNA polymorphisms

PCR technique used to generate strain-specific arrays of anonymous DNA fragments

thumb|An amplicon sequence template that has been prepared for amplification. The target sequence to be amplified is colored green. In molecular biology, an amplicon is a piece of DNA that is the source and/or product of amplification or replication events. It can be formed artificially, using various methods including polymerase chain reactions (PCR) or ligase chain reactions (LCR), or naturally through gene duplication. In this context, amplification refers to the production of one or more copies of a genetic fragment or target sequence, specifically the amplicon. As it refers to the product

type of PCR

method of DNA replication

TaqMan probes are hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher David Gefland at Cetus Corporation, and the technology was subsequently developed by Hoffmann-La Roche for diagnostic assays and by Applied Biosystems (now part of Thermo Fisher Scientific) for research applications.

group of protein complexes

class of enzymes

use of polymerase chain reaction to amplify several different DNA

multiplex ligation-dependent probe amplification

a theoretical value, which is used in molecular biology

biotechnological procedure

technique for finding unknown DNA flanking known DNA