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American lesbian actresses

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Ellen DeGeneres
American comedian, television host, actress, and writer (born 1958)
Chloë Grace Moretz
Chloë Grace Moretz is an American actress. She began acting as a child, with early roles in the horror film The Amityville Horror (2005), the drama series Desperate Housewives (2006–2007), the horror film The Eye (2008), the drama film The Poker House (2008), the romantic comedy film 500 Days of Summer (2009), and the children's comedy film Diary of a Wimpy Kid (2010). Her breakthrough came in 2010 with her performance as Hit-Girl in the superhero film Kick-Ass.
Lily Tomlin
American actress, comedian and writer
Portia de Rossi
Australian-American actress
Jane Lynch
American actress
Rachel Maddow
American television news host and political commentator
Rosie O'Donnell
American comedian, producer, actress, and television personality
Linda Hunt
American actress
Kate McKinnon
American actress and comedian (born 1984)
Katherine Moennig
American actress (born 1977)
Holland Taylor
American actress
Kelly McGillis
American actress
Lesley Gore
American singer (1946–2015)
Sara Gilbert
American actress
Nikki Blonsky
American actress and singer
Cherry Jones
American actress
Wanda Sykes
American comedian, writer, actress and voice artist
Hayley Kiyoko
American singer and actress
Diora Baird
American actress and model
Amandla Stenberg
American actress
Clea DuVall
American actress
Amanda Bearse
American actress, comedian and director (born 1958)
Bree Olson
former American pornographic actress (born 1986)
Jean Acker
American actress (1893–1978)
Sasha Lane
American actress
Fran Lebowitz
Frances Ann Lebowitz is an American author, public speaker, cultural critic, and actor. She is known for her sardonic social commentary on American life as filtered through her New York City sensibilities and her association with many prominent figures of the New York art scene of the 1970s and 1980s, including Andy Warhol, Martin Scorsese, Jerome Robbins, Robert Mapplethorpe, David Wojnarowicz, Candy Darling, and the New York Dolls.
Kristy McNichol
American actress
Meredith Baxter
American actress and producer (born 1947)
Maude Adams
American actress (1872–1953)
Valorie Curry
American actress
Eva Le Gallienne
British-American actress and author (1899–1991)
Lena Waithe
Lena Waithe is an American actress, producer, and screenwriter. She is the creator of the Showtime drama series The Chi (2018–present) and the BET comedy series Boomerang (2019–20) and Twenties (2020–21). She also wrote and produced the crime film Queen & Slim (2019) and is the executive producer of the horror anthology series Them (2021–present).
Susan Flannery
American actress
Leisha Hailey
American singer, songwriter and actress (born 1971)
Heather Matarazzo
American actress
Alexandra Hedison
American actress and photographer
Lea DeLaria
American actor, comedian and singer
Margaret Lindsay
American actress (1910-1981)
Dot Jones
athlete, actor
Emily Rios
American actress
Jasika Nicole
American actress
Reneé Rapp
American actress and singer
Guinevere Turner
American actress and writer
Katharine Cornell
American actress, theatre manager and producer (1893-1974)
Patsy Kelly
American actress (1910–1981)
Cheryl Dunye
American actress and director (born 1966)
Katy O'Brian
American actress, writer and martial artist
Charlotte Cushman
American actress (1816–1876)
Maile Flanagan
American actress
Tig Notaro
American stand-up comic, writer, podcaster, and actress
Lisa Banes
American actress (1955–2021)
Jenna von Oy
American actress
Jasmin Savoy Brown
American actress
Jenny Shimizu
American model and actress
Hannah Hart
American Internet celebrity
Sasheer Zamata
American actress and comedian
Grace Darmond
American actress (1893–1963)
Nancy Kulp
actress (1921-1991)
Fortune Feimster
American comedian, actor, and writer
Barbara Dare
American pornographic actress