Plasmids

circular plasmid used in creation of transgenic plants

small piece of DNA that can be stably maintained in an organism for cloning

The F-plasmid (first named F by one of its discoverers Esther Lederberg；also called the sex factor in E. coli，the F sex factor, the fertility factor, or simply the F factor) allows genes to be transferred from one bacterium carrying the factor to another bacterium lacking the factor by conjugation. The F factor was the first plasmid to be discovered. Unlike other plasmids, F factor is constitutive for transfer proteins due to a mutation in the gene finO. The F plasmid belongs to F-like plasmids, a class of conjugative plasmids that control sexual functions of bacteria with a fertility inhibiti

plasmid specifically used for protein expression in the target

thumb|A schematic representation of the pBR322 vector with restriction sites indicated in blue. pBR322 is a plasmid and was one of the first widely used cloning vectors for E. coli. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after Francisco Bolívar Zapata, a postdoctoral researcher, and Raymond L. Rodriguez. The p stands for "plasmid," and BR for "Bolivar" and "Rodriguez."

biological process

The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene. GFP was isolated from the jelly fish Aequorea victoria. Because it shares a bidirectional promoter with a gene for metabolizing arabinose, the GFP gene is expressed in the presence of arabinose, which makes the transgenic organism express its fluorescence under UV light. GFP can be induced in bacteria containing the pGLO plasmid by growing them on