thumb|A schematic representation of the pBR322 vector with restriction sites indicated in blue. pBR322 is a plasmid and was one of the first widely used cloning vectors for E. coli. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after Francisco Bolívar Zapata, a postdoctoral researcher, and Raymond L. Rodriguez. The p stands for "plasmid," and BR for "Bolivar" and "Rodriguez."
thumb|A schematic representation of the pBR322 vector with restriction sites indicated in blue. pBR322 is a plasmid and was one of the first widely used cloning vectors for E. coli. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after Francisco Bolívar Zapata, a postdoctoral researcher, and Raymond L. Rodriguez. The p stands for "plasmid," and BR for "Bolivar" and "Rodriguez."
pBR322 is 4361 base pairs in length and has two antibiotic resistance genes: blaTEM-1, which confers ampicillin resistance, and tetA, which confers tetracycline resistance. It contains the origin of replication of pMB1, and the rop gene, which encodes a restrictor of plasmid copy number. The plasmid has unique restriction sites for more than forty restriction enzymes. Eleven of these forty sites lie within the tetA gene. There are two restriction sites, HindIII and ClaI, within the promoter of the tetA gene. There are six key restriction sites inside the blaTEM-1 gene. The antibiotic resistance genes are from pSC101 for tetracycline and RSF2124 for ampicillin. The restriction sites in the antibiotic resistance genes allow for insertional inactivation.
Discovered by embedding cosine similarity (sentence-transformers MiniLM, 384-dim).