Key facts
- Ec no
- 6.5.1.1
- Cas no
- 9015-85-4
- Intenz
- IntEnz view
- Brenda
- BRENDA entry
- Expasy
- NiceZyme view
- Kegg
- KEGG entry
- Metacyc
- metabolic pathway
- Priam
- profile
- Pdb structures
- RCSB PDB PDBe PDBsum
- Gene ontology
- AmiGO / QuickGO
- Pmc
- articles
- Pubmed
- articles
- Ncbi
- proteins
via Wikipedia infobox
Research
22,594 papers- Assembling Multiple Fragments: The Gibson Assembly.Methods in molecular biology (Clifton, N.J.) · 2023
- DNA ligase I, the replicative DNA ligase.Sub-cellular biochemistry · 2012
- R2D ligase: Unveiling a novel DNA ligase with surprising DNA-to-RNA ligation activity.Biotechnology journal · 2024
- Rare variants of DNA ligase 1 show distinct mechanisms of deficiency.The Journal of biological chemistry · 2024
- Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes.Nature communications · 2023
via PubMed
~14 min read
Encyclopedic overview
DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA). Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.
DNA ligase is used in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments (see Research applications). Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA.
Excerpted from Wikipedia’s “DNA ligase” article, available under the CC BY-SA 4.0 licence.