immunoprecipitation
Sign in to saveImmunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.
Research
180,441 papers- [Advances in phage immunoprecipitation sequencing technology].Sheng wu gong cheng xue bao = Chinese journal of biotechnology · 2025
- Immunoprecipitation of HDAC6 and Interacting Proteins.Methods in molecular biology (Clifton, N.J.) · 2023
- Methyl DNA immunoprecipitation.Methods in molecular biology (Clifton, N.J.) · 2009
- Using Chromatin Immunoprecipitation (ChIP) to Study the Chromatin State in Drosophila.Cold Spring Harbor protocols · 2025
- Chromatin Immunoprecipitation on Fixed Tissues and Cell Lines.Methods in molecular biology (Clifton, N.J.) · 2023
via PubMed
Article
25 sectionsContents
- Types
- Individual protein immunoprecipitation (IP)
- Protein complex immunoprecipitation (Co-IP)
- Chromatin immunoprecipitation (ChIP)
- RNP immunoprecipitation (RIP and CLIP)
- Tagged proteins
- Methods
- Direct
- Indirect
- Selection
- Technological advances
- Agarose
- Preclearing
- Superparamagnetic beads
- Agarose vs. magnetic beads
- Binding capacity
- Cost
- Equipment
- Automation
- Summary
- Protocol
- Background
- Steps
- References
- External links
Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.
== Types ==