resazurin
Sign in to saveResazurin (7-Hydroxy-3H-phenoxazin-3-one 10-oxide) is a phenoxazine dye that is weakly fluorescent, nontoxic, cell-permeable, and redox‐sensitive. Resazurin has a blue to purple color above pH 6.5 and an orange color below pH 3.8. It is used in microbiological, cellular, and enzymatic assays because it can be irreversibly reduced to the pink-colored and highly fluorescent resorufin (7-Hydroxy-3H-phenoxazin-3-one). At circum-neutral pH, resorufin can be detected by visual observation of its pink color or by fluorimetry, with an excitation maximum at 530-570 nm and an emission maximum at 58
In the Vinony graph
Within Vinony's link graph, resazurin is referenced by 21 other articles, and connects out to cellular respiration, fluorescence and reduction potential.
Vinony files it under Amine oxides, Cell culture reagents and Chembox having GHS data.
Its subject is documented across 16 Wikipedia language editions.
Research
2,621 papers- Standard Operating Procedure to Optimize Resazurin-Based Viability Assays.Biosensors · 2024
- Basic Colorimetric Proliferation Assays: MTT, WST, and Resazurin.Methods in molecular biology (Clifton, N.J.) · 2017
- Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity.European journal of biochemistry · 2000
- Resazurin dye is an in vivo sensor of kidney tubular function.Kidney international · 2025
- Resazurin microplate test method for rapid determination of colistin resistance in carbapenem-resistant Acinetobacter baumanii isolates.Laboratory medicine · 2024
via PubMed
Wikidata facts
- Mass
- 229.038
Show 3 more facts
- canonical SMILES
- C1=CC2=C(C=C1O)OC3=CC(=O)C=CC3=[N+]2[O-]
- chemical formula
- C₁₂H₇NO₄
- Commons category
- Resazurin
via Wikidata · CC0
~6 min read
Encyclopedic overview
6 sectionsContents
- Cell viability applications
- Resazurin reduction test
- Other applications
- Synthesis
- Related dyes
- References
Resazurin (7-Hydroxy-3H-phenoxazin-3-one 10-oxide) is a phenoxazine dye that is weakly fluorescent, nontoxic, cell-permeable, and redox‐sensitive. Resazurin has a blue to purple color above pH 6.5 and an orange color below pH 3.8. It is used in microbiological, cellular, and enzymatic assays because it can be irreversibly reduced to the pink-colored and highly fluorescent resorufin (7-Hydroxy-3H-phenoxazin-3-one). At circum-neutral pH, resorufin can be detected by visual observation of its pink color or by fluorimetry, with an excitation maximum at 530-570 nm and an emission maximum at 580-590 nm.
When a solution containing resorufin is submitted to reducing conditions (Eh h of this same solution is increased, dihydroresorufin is oxidized back to resorufin, and this reversible reaction can be used to monitor if the redox potential of a culture medium remains at a sufficiently low level for anaerobic organisms.
Excerpted from Wikipedia’s “resazurin” article, available under the CC BY-SA 4.0 licence.