~4 min read
Article
4 sectionsContents
- Gel zymography
- Variations on the standard protocol
- See also
- References
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Zymography is an electrophoretic technique for the detection of hydrolytic enzymes, based on the substrate repertoire of the enzyme. Three types of zymography are used; in gel zymography, in situ zymography and in vivo zymography. For instance, gelatin embedded in a polyacrylamide gel will be digested by active gelatinases run through the gel. After Coomassie staining, areas of degradation are visible as clear bands against a darkly stained background.
Connections
International Standard Book Number
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enzyme
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digital object identifier
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agar
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hydrolysis
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Q180686
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gelatin
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Drosophila melanogaster
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bibcode
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casein
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electrophoresis
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sodium lauryl sulfate
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Q22908627
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enzyme inhibitor
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gel electrophoresis
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Handle System
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Coomassie Brilliant Blue
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dithiothreitol
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SDS-PAGE
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glycoside hydrolase superfamily
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