~4 min read
Encyclopedic overview
4 sectionsContents
- Gel zymography
- Variations on the standard protocol
- See also
- References
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Zymography is an electrophoretic technique for the detection of hydrolytic enzymes, based on the substrate repertoire of the enzyme. Three types of zymography are used; in gel zymography, in situ zymography and in vivo zymography. For instance, gelatin embedded in a polyacrylamide gel will be digested by active gelatinases run through the gel. After Coomassie staining, areas of degradation are visible as clear bands against a darkly stained background.
Excerpted from Wikipedia’s “Zymography” article, available under the CC BY-SA 4.0 licence.