half maximal inhibitory concentration
thumb|Graphical representation of the IC50 determination of the inhibition of an enzyme's activity by a small molecule ("drug"). Four different concentrations of the small molecule (ranging from 30 to 300 μM) were tested.
Research
8,222 papers- Calculating Half Maximal Inhibitory Concentration (IC(50)) Values from Glycomics Microarray Data Using GraphPad Prism.Methods in molecular biology (Clifton, N.J.) · 2022
- Determination of Half-Maximal Inhibitory Concentration of an Enzyme Inhibitor.Methods in molecular biology (Clifton, N.J.) · 2020
- Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action.Virulence · 2018
- Pharmacological Characterization of Zasocitinib (TAK-279): An Oral, Highly Selective, and Potent Allosteric TYK2 Inhibitor.The Journal of investigative dermatology · 2026
- Assessing Drug Sensitivity in Fission Yeast Using Half-Maximal Inhibitory Concentration (IC50) Assays.Methods in molecular biology (Clifton, N.J.) · 2025
via PubMed
Article
7 sectionsContents
- Functional antagonist assay
- IC<sub>50</sub> and affinity
- Competition binding assays
- Cheng–Prusoff equation
- See also
- References
- External links
thumb|Graphical representation of the IC50 determination of the inhibition of an enzyme's activity by a small molecule ("drug"). Four different concentrations of the small molecule (ranging from 30 to 300 μM) were tested.
Half maximal inhibitory concentration (IC50) is a measure of the potency of a substance in inhibiting a specific biological or biochemical function. IC50 is a quantitative measure that indicates how much of a particular inhibitory substance (e.g. drug) is needed to inhibit, in vitro, a given biological process or biological component by 50%. The biological component could be an enzyme, cell, cell receptor or microbe. IC50 values are typically expressed as molar concentration.