HL-60
Sign in to saveAlso known as HL 60, HL.60, HL60, Human Leukemia-60
The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36–48 hours. The cell line was derived from a 36-year-old woman who was originally reported to have acute promyelocytic leukemia at the MD Anderson Cancer Center. HL-60 cells predominantly show neutrophilic promyelocytic morphology. Subsequent evaluation, including the karyotype that showed absence of the defining t(15;17) translocation, conclude
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Vinony's link graph records 32 inbound references to HL-60, and connects out to calcium, antibiotic and digital object identifier.
Vinony files it under Cancer cell lines, Human cell lines and Leukemia cell lines.
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Research
20,170 papers- Use of HL-60 cell line to measure opsonic capacity of pneumococcal antibodies.Clinical and diagnostic laboratory immunology · 2005
- Living and dying with reactive species. Focus on "peroxynitrite induces apoptosis of HL-60 cells by activation of a caspase-3 family protease".The American journal of physiology · 1998
- HL-60 cells degrade alpha-actinin to produce a fragment that promotes monocyte/macrophage maturation.Experimental hematology · 1999
- Protocol for the differentiation of HL-60 cells into a neutrophil-like state.STAR protocols · 2025
- Mechanism of the eosinophilic differentiation of HL-60 clone 15 cells induced by n-butyrate.International archives of allergy and immunology · 2005
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- Anaplasma phagocytophilum cultured in human promyelocytic cell line HL-60.jpg
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- HL-60 cells
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- purl.obolibrary.org/obo/CLO_0050972
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Encyclopedic overview
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The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36–48 hours. The cell line was derived from a 36-year-old woman who was originally reported to have acute promyelocytic leukemia at the MD Anderson Cancer Center. HL-60 cells predominantly show neutrophilic promyelocytic morphology. Subsequent evaluation, including the karyotype that showed absence of the defining t(15;17) translocation, concluded that HL-60 cells are from a case of AML FAB-M2 (now referred to as AML with maturation (WHO)).
Proliferation of HL-60 cells occurs through the transferrin and insulin receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this line, differentiation to mature granulocytes can be induced by compounds such as dimethyl sulfoxide (DMSO), or retinoic acid. Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 to differentiate to monocytic, macrophage-like and eosinophil phenotypes, respectively.
Excerpted from Wikipedia’s “HL-60” article, available under the CC BY-SA 4.0 licence.