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ABTS
Sign in to saveAlso known as A2BTS, C18H18N4O6S4
In biochemistry, ABTS ('''2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)''') is a chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect the binding of molecules to each other.
Chemical data
- Formula
- C18H18N4O6S4
- Molecular weight
- 514.6 g/mol
- IUPAC name
- (2E)-3-ethyl-2-[(Z)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid
- SMILES
- CCN\1C2=C(C=C(C=C2)S(=O)(=O)O)S/C1=N/N=C\3/N(C4=C(S3)C=C(C=C4)S(=O)(=O)O)CC
- InChIKey
- ZTOJFFHGPLIVKC-YAFCTCPESA-N
- XLogP
- 2.5
- Polar surface area
- 207 Ų
- H-bond donors
- 2
- H-bond acceptors
- 10
- Formal charge
- 0
via PubChem
Research
16,393 papers- Antioxidant activity applying an improved ABTS radical cation decolorization assay.Free radical biology & medicine · 1999
- Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins.International journal of biological macromolecules · 2023
- ABTS/PP Decolorization Assay of Antioxidant Capacity Reaction Pathways.International journal of molecular sciences · 2020
- Enhanced chlorination of diclofenac using ABTS as electron shuttle: Performance, mechanism and applicability.The Science of the total environment · 2024
- Electrochemically-functionalized CNT/ABTS nanozyme enabling sensitive and selective voltammetric detection of microalbuminuria.Analytica chimica acta · 2022
via PubMed
Wikidata facts
- Subclass of
- chemical compound
- Mass
- 548.064016488
Show 5 more facts
- chemical formula
- C₁₈H₁₈N₄O₆S₄
- canonical SMILES
- CCN1/C(Sc2cc(ccc12)S(O)(=O)=O)=N/N=C/3Sc4cc(ccc4N3CC)S(O)(=O)=O
- different from
- heavy rescue vehicle
- Commons category
- ABTS
- isomeric SMILES
- CCn1/c(=N/N=c2\sc3cc(S(=O)(=O)O)ccc3n2CC)sc2cc(S(=O)(=O)O)ccc21.N.N
Sources (3)
via Wikidata · CC0
~3 min read
Encyclopedic overview
2 sectionsContents
- Applications for functional food analysis
- References
In biochemistry, ABTS ('''2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)''') is a chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect the binding of molecules to each other.
It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme (such as horseradish peroxidase) or alone with blue multicopper oxidase enzymes (such as laccase or bilirubin oxidase). Its use allows the reaction kinetics of peroxidases themselves to be followed. In this way it also can be used to indirectly follow the reaction kinetics of any hydrogen peroxide-producing enzyme, or to simply quantify the amount of hydrogen peroxide in a sample.
Excerpted from Wikipedia’s “ABTS” article, available under the CC BY-SA 4.0 licence.
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