File:HPLC_Instrument.jpg · Wikimedia Commons · See Wikimedia Commons
high-performance liquid chromatography
Sign in to saveAlso known as high-pressure liquid chromatography, Chromatography, High Pressure Liquid
method
Research
276,346 papers- High-performance liquid chromatography of porphyrins.Journal of chromatography · 1988
- High-performance liquid chromatography of mono- and oligosaccharides.Analytical biochemistry · 1984
- Size exclusion-high-performance liquid chromatography (SEC-HPLC).Methods in molecular biology (Clifton, N.J.) · 2014
- [High performance liquid chromatography and its clinical use].Nihon rinsho. Japanese journal of clinical medicine · 1981
- High performance liquid chromatography and related methods in purification of monoclonal antibodies.Advances in biotechnological processes · 1989
via PubMed
~40 min read
Encyclopedic overview
A modern self-contained HPLC Schematic representation of an HPLC unit (1) solvent reservoirs, (2) solvent degasser, (3) gradient valve, (4) mixing vessel for delivery of the mobile phase, (5) high-pressure pump, (6) switching valve in "inject position", (6') switching valve in "load position", (7) sample injection loop, (8) pre-column (guard column), (9) analytical column, (10) detector (i.e., IR, UV), (11) data acquisition, (12) waste or fraction collector.
High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify specific components in mixtures. The mixtures can originate from food, chemicals, pharmaceuticals, biological, environmental and agriculture, etc., in which the sample analyzed is either a liquid or has been dissolved into a liquid.
Excerpted from Wikipedia’s “high-performance liquid chromatography” article, available under the CC BY-SA 4.0 licence.