EntityQ381233· pop 45· linked from 889 articles

high-performance liquid chromatography

method

Research

276,346 papers

High-performance liquid chromatography of porphyrins.Journal of chromatography · 1988
High-performance liquid chromatography of mono- and oligosaccharides.Analytical biochemistry · 1984
Size exclusion-high-performance liquid chromatography (SEC-HPLC).Methods in molecular biology (Clifton, N.J.) · 2014
[High performance liquid chromatography and its clinical use].Nihon rinsho. Japanese journal of clinical medicine · 1981
High performance liquid chromatography and related methods in purification of monoclonal antibodies.Advances in biotechnological processes · 1989

Article

A modern self-contained HPLC Schematic representation of an HPLC unit (1) solvent reservoirs, (2) solvent degasser, (3) gradient valve, (4) mixing vessel for delivery of the mobile phase, (5) high-pressure pump, (6) switching valve in "inject position", (6') switching valve in "load position", (7) sample injection loop, (8) pre-column (guard column), (9) analytical column, (10) detector (i.e., IR, UV), (11) data acquisition, (12) waste or fraction collector.

High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify specific components in mixtures. The mixtures can originate from food, chemicals, pharmaceuticals, biological, environmental and agriculture, etc., in which the sample analyzed is either a liquid or has been dissolved into a liquid.