molecular cloning
Sign in to saveAlso known as cloning, molecular, DNA cloning, DNA Cloning
insertion of recombinant DNA molecules, by means of a replicating vehicle, into recipient cells without altering their viability
Research
214,776 papers- Molecular cloning.Methods in enzymology · 2013
- Classical Recombinant DNA Cloning.Methods in molecular biology (Clifton, N.J.) · 2023
- Molecular cloning.Biotechnology (Reading, Mass.) · 1992
- Phosphate transport: from microperfusion to molecular cloning.Pflugers Archiv : European journal of physiology · 2019
- Assembling Multiple Fragments: The Gibson Assembly.Methods in molecular biology (Clifton, N.J.) · 2023
via PubMed
~22 min read
Encyclopedic overview
Diagram of molecular cloning using bacteria and plasmids
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.
Excerpted from Wikipedia’s “molecular cloning” article, available under the CC BY-SA 4.0 licence.