cytochemistry

right|thumb|400 px|Transmission electron microscope|Transmission electron micrograph of a [[chondrocyte, stained for calcium, showing its nucleus (N) and mitochondria (M).]] Cytochemistry is the branch of cell biology dealing with the detection of cell constituents by means of biochemical analysis and visualization techniques. This is the study of the localization of cellular components through the use of staining methods. The term is also used to describe a process of identification of the biochemical content of cells. Cytochemistry is a science of localizing chemical components of cells and cell organelles on thin histological sections by using several techniques like enzyme localization, micro-incineration, micro-spectrophotometry, radioautography, cryo-electron microscopy, X-ray microanalysis by energy-dispersive X-ray spectroscopy, immunohistochemistry and cytochemistry, etc.

== Freeze fracture enzyme cytochemistry == Freeze fracture enzyme cytochemistry was initially mentioned in the study of Pinto de silva in 1987. It is a technique that allows the introduction of cytochemistry into a freeze fracture cell membrane. immunocytochemistry is used in this technique to label and visualize the cell membrane's molecules. This technique could be useful in analyzing the ultrastructure of cell membranes. The combination of immunocytochemistry and freeze fracture enzyme technique, research can identify and have a better understanding of the structure and distribution of a cell membrane.