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Immunofixation
Sign in to savethumb|Pipetting anti-immunoglobulins to immunofixation panel. The panel simultaneously tests 4 patients (one in each quadrant). Each patient has 6 electrophoresis panels: The left one is a conventional serum protein electrophoresis. The remainder get solutions with anti-IgG, anti-IgA, anti-IgM, anti-kappa light chain and anti-lambda light chain immunoglobulin, respectively from left to right. Each anti-immunoglobulin solution is artificially colored to ensure that the solution matches the color map at top. thumb|Immunofixation electrophoresis, schematic representation:- A. Normal serum- B. Mon
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- Principle
- Technique
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thumb|Pipetting anti-immunoglobulins to immunofixation panel. The panel simultaneously tests 4 patients (one in each quadrant). Each patient has 6 electrophoresis panels: The left one is a conventional serum protein electrophoresis. The remainder get solutions with anti-IgG, anti-IgA, anti-IgM, anti-kappa light chain and anti-lambda light chain immunoglobulin, respectively from left to right. Each anti-immunoglobulin solution is artificially colored to ensure that the solution matches the color map at top. thumb|Immunofixation electrophoresis, schematic representation:- A. Normal serum- B. Monoclonal intact immunoglobulin IgGλ- C, D. Monoclonal intact immunoglobulin IgDλ and free light chain λ (Fλ).Con. = Conventional electrophoresis staining of the total protein. Immunofixation (IFX, also called immunofixation electrophoresis or IFE) permits the simultaneous detection and typing of monoclonal antibodies (immunoglobulins) in serum or urine. It is of great importance for the diagnosis and monitoring of monoclonal gammopathies.
== Principle ==
Excerpted from Wikipedia’s “Immunofixation” article, available under the CC BY-SA 4.0 licence.