immunohistochemistry
Sign in to savethumb|230px|Main staining patterns on chromogenic immunohistochemistry. thumb|right|Immunofluorescence of human skin using an anti-IgA antibody. The skin is from a patient with [[Henoch–Schönlein purpura: IgA deposits are found in the walls of small superficial capillaries (yellow arrows). The pale wavy green area on top is the epidermis, the bottom fibrous area is the dermis.]] thumb|"Block" staining: strong nuclear and cytoplasmic expression in a continuous segment of cells. Immunohistochemistry is a form of immunostaining. It involves the process of selectively identifying antigens in cells
Article
20 sectionsContents
- Sample preparation
- Tissue preparation and fixation
- Sectioning
- Antigen retrieval
- Blocking
- Sample labeling
- Antibody types
- Detection methods
- Reporter molecules
- Counterstains
- Troubleshooting
- Diagnostic immunohistochemistry markers
- Directing therapy
- Chemical inhibitors
- Monoclonal antibodies
- Mapping protein expression
- See also
- References
- Further reading
- External links
thumb|230px|Main staining patterns on chromogenic immunohistochemistry. thumb|right|Immunofluorescence of human skin using an anti-IgA antibody. The skin is from a patient with [[Henoch–Schönlein purpura: IgA deposits are found in the walls of small superficial capillaries (yellow arrows). The pale wavy green area on top is the epidermis, the bottom fibrous area is the dermis.]] thumb|"Block" staining: strong nuclear and cytoplasmic expression in a continuous segment of cells. Immunohistochemistry is a form of immunostaining. It involves the process of selectively identifying antigens in cells and tissue, by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Albert Hewett Coons, Ernest Berliner, Norman Jones and Hugh J Creech was the first to develop immunofluorescence in 1941. This led to the later development of immunohistochemistry.
Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. In some cancer cells certain tumor antigens are expressed which make it possible to detect. Immunohistochemistry is also widely used in basic research, to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.