spheroplast
Sign in to savethumb|upright|right|Gram-negative bacteria attempting to grow and divide in the presence of peptidoglycan synthesis-inhibiting antibiotics (e.g. penicillin) fail to do so, and instead end up forming spheroplasts. A spheroplast (or sphaeroplast in British usage) is a microbial cell from which the cell wall has been almost completely removed, as by the action of penicillin or lysozyme. According to some definitions, the term is used to describe Gram-negative bacteria. According to other definitions, the term also encompasses yeasts. The name spheroplast stems from the fact that after the microbe
Research
2,726 papers- Spheroplast-Mediated Carbapenem Tolerance in Gram-Negative Pathogens.Antimicrobial agents and chemotherapy · 2019
- Rescue of Infectious Sindbis Virus by Yeast Spheroplast-Mammalian Cell Fusion.Viruses · 2021
- Recovery of Vibrio cholerae polarized cellular organization after exit from a non-proliferating spheroplast state.PloS one · 2023
- Spheroplast fusion and heterokaryon formation in Mucor racemosus.Journal of bacteriology · 1978
- Characterization of spheroplast membranes of Neisseria meningitidis group B.Infection and immunity · 1972
via PubMed
Wikidata facts
- Image
- Patchclamp Spheroplast1.jpg
Sources (3)
via Wikidata · CC0
~5 min read
Article
11 sectionsContents
- Spheroplast formation
- Antibiotic-induced spheroplasts
- Enzyme-induced spheroplasts
- Uses and applications
- Antibiotic discovery
- Patch clamping
- Cell lysis
- Transfection
- See also
- References
- External links
thumb|upright|right|Gram-negative bacteria attempting to grow and divide in the presence of peptidoglycan synthesis-inhibiting antibiotics (e.g. penicillin) fail to do so, and instead end up forming spheroplasts. A spheroplast (or sphaeroplast in British usage) is a microbial cell from which the cell wall has been almost completely removed, as by the action of penicillin or lysozyme. According to some definitions, the term is used to describe Gram-negative bacteria. According to other definitions, the term also encompasses yeasts. The name spheroplast stems from the fact that after the microbe's cell wall is digested, membrane tension causes the cell to acquire a characteristic spherical shape. Spheroplasts are osmotically fragile, and will lyse if transferred to a hypotonic solution.
When used to describe Gram-negative bacteria, the term spheroplast refers to cells from which the peptidoglycan component but not the outer membrane component of the cell wall has been removed.