دیایپیآی
Also known as 4',6-diamidino-2-phenylindole, antifade, 2-(4-(aminoiminomethyl)phenyl)-1H-indole-6-carboximidamide, 4',6-diamidinophenyl-indole
DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore provides a marker for membrane viability.
Chemical data
- Formula
- C16H15N5
- Molecular weight
- 277.32 g/mol
- IUPAC name
- 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide
- SMILES
- C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N
- InChIKey
- FWBHETKCLVMNFS-UHFFFAOYSA-N
- XLogP
- 1.6
- Polar surface area
- 116 Ų
- H-bond donors
- 5
- H-bond acceptors
- 2
- Formal charge
- 0
via PubChem