DAPI

Also known as 4',6-diamidino-2-phenylindole, antifade, 2-(4-(aminoiminomethyl)phenyl)-1H-indole-6-carboximidamide, 4',6-diamidinophenyl-indole

marqueur bleu fluorescent se fixant sur l'ADN des cellules

Chemical data

Formula: C16H15N5
Molecular weight: 277.32 g/mol
IUPAC name: 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide
SMILES: C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N
InChIKey: FWBHETKCLVMNFS-UHFFFAOYSA-N
XLogP: 1.6
Polar surface area: 116 Å²
H-bond donors: 5
H-bond acceptors: 2
Formal charge: 0

via PubChem

Research

10,023 papers

DAPI: a DNA-specific fluorescent probe.Biotechnic & histochemistry : official publication of the Biological Stain Commission · 1995
Labeling nuclear DNA using DAPI.Cold Spring Harbor protocols · 2011
DAPI (4',6-diamidino-2-phenylindole) Stains Compact Amyloid Plaques.Journal of Alzheimer's disease : JAD · 2022
CMA/DAPI Banding of Plant Chromosomes.Methods in molecular biology (Clifton, N.J.) · 2023
A cautionary (spectral) tail: red-shifted fluorescence by DAPI-DAPI interactions.Biochemical Society transactions · 2016

via PubMed

Wikidata facts

Mass: 277.133
Image: Indian Muntjac fibroblast cells (24271618921).jpg

Show 3 more facts

Commons category: DAPI
chemical formula: C₁₆H₁₅N₅
canonical SMILES: C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N

Sources (4)

via Wikidata · CC0

Article · Français

Le DAPI ou 4',6-diamidino-2-phénylindole est une molécule fluorescente capable de se lier fortement aux bases adénine (A) et thymine (T) de l'ADN. Le DAPI est un fluorochrome : quand le DAPI absorbe la lumière UV (350 nm), il émet une fluorescence bleue brillante (450-490 nm) , ce qui permet de détecter et quantifier l'ADN grâce à un microscope à fluorescence ou par cytométrie en flux.

Abstract from DBpedia / Wikipedia · CC BY-SA