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green fluorescent protein
Sign in to saveAlso known as green fluorescent proteins
protein that converts blue and ultraviolet light ranges to green light
OverviewAI-generated
Green fluorescent protein is a protein found in the water jellyfish *Aequorea victoria*. The gene encoding this protein is designated as GFP. Its UniProt accession number is P42212. The protein is classified as a photoprotein and exhibits luminescence. It contains a chromophore and possesses a defined three-dimensional structure.
The existence of green fluorescent protein is supported by evidence at the protein level. The protein has been subjected to direct protein sequencing. These attributes are associated with the subject in scientific databases, which contain 74,458 PubMed entries related to the query.
Synthesized by Vinony from 9 facts across 2 sources: PubMed, UniProt. Generated from structured data (not the Wikipedia text) and checked against those facts — may still contain errors.
Key facts
- Symbol
- GFP
- Pfam
- PF01353
- Pfam clan
- CL0069
- Interpro
- IPR011584
- Cath
- 1ema
- Scop2
- 1ema / SCOPe / SUPFAM
- Pdb
- IPR011584 PF01353 ( ECOD ; PDBsum )
- Alphafold
- IPR011584 PF01353
via Wikipedia infobox
Protein · UniProt
Green fluorescent protein
- Gene
- GFP
- Organism
- Aequorea victoria (Water jellyfish)
- Length
- 238 aa
- Molecular mass
- 26,886 Da
- Evidence
- 1: Evidence at protein level
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin
Swiss-Prot (reviewed) · via UniProt
Research
74,458 papers- Green fluorescent protein: a perspective.Protein science : a publication of the Protein Society · 2011
- Green fluorescent protein inspired fluorophores.Advances in colloid and interface science · 2020
- Engineering and characterization of a superfolder green fluorescent protein.Nature biotechnology · 2006
- RNA mimics of green fluorescent protein.Science (New York, N.Y.) · 2011
- A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.Nature methods · 2013
via PubMed
Wikidata facts
- Instance of
- protein
Show 3 more facts
- has characteristic
- fluorescence
- Commons category
- Green fluorescent proteins
- found in taxon
- Medusa T Pasas de Verga
Sources (3)
via Wikidata · CC0
~31 min read
Encyclopedic overview
The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP. However, GFPs have been found in other organisms including corals, sea anemones, zoanithids, copepods and lancelets.
The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form an internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.
Excerpted from Wikipedia’s “green fluorescent protein” article, available under the CC BY-SA 4.0 licence.