pyrosequencing
Sign in to savePyrosequencing is a non-electrophoretic DNA sequencing (determining the order of nucleotides in DNA) method based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released, hence, the name given it.
Article
6 sectionsContents
- Principles<!-- I would transform this section in "History", imrpoving the chronology of discovery and commercialization of the method, while the detailed principle can go in the next session. -->
- Procedure
- Limitations
- Commercialization
- Further reading
- References
Pyrosequencing is a non-electrophoretic DNA sequencing (determining the order of nucleotides in DNA) method based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released, hence, the name given it.
==Principles==