Safranin
Safranin (Safranin O or basic red 2) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring cell nuclei red. This is the classic counterstain in both Gram stains and endospore staining. It can also be used for the detection of cartilage, mucin and mast cell granules.
Chemical data
- Formula
- C20H19ClN4
- Molecular weight
- 350.8 g/mol
- IUPAC name
- 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine chloride
- SMILES
- CC1=CC2=C(C=C1N)[N+](=C3C=C(C(=CC3=N2)C)N)C4=CC=CC=C4.[Cl-]
- InChIKey
- OARRHUQTFTUEOS-UHFFFAOYSA-N
- Polar surface area
- 68.8 Ų
- H-bond donors
- 2
- H-bond acceptors
- 4
- Formal charge
- 0
via PubChem