3,3'-diaminobenzidine

Also known as 3,3',4,4'-tetraminobiphenyl, biphenyl-3,3',4,4'-tetrayltetraamine, 3,3',4,4'-biphenyltetramine, 3,3',4,4'-tetraaminobiphenyl, (1,1'-biphenyl)-3,3',4,4'-tetramine, 3,3',4,4'-tetraaminodiphenyl, 3,3'-diaminobenzidene, 3,3',4,4'-diphenyltetramine

3,3′-Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. This derivative of benzidine is a precursor to polybenzimidazole, which forms fibers that are renowned for their chemical and thermal stability. As its water-soluble tetrahydrochloride, DAB has been used in immunohistochemical staining of nucleic acids and proteins.

Research

1,502 papers

3,3'-Diaminobenzidine staining interferes with PCR-based DNA analysis.Scientific reports · 2018
Inhibition of catalase by 3,3'-diaminobenzidine.The Biochemical journal · 1985
Histochemistry and cytochemistry of 3,3'-diaminobenzidine. A review.Folia histochemica et cytochemica · 1979
DAB-quant: An open-source digital system for quantifying immunohistochemical staining with 3,3'-diaminobenzidine (DAB).PloS one · 2022
Determination and detection of reactive oxygen species (ROS), lipid peroxidation, and electrolyte leakage in plants.Methods in molecular biology (Clifton, N.J.) · 2010

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Article

5 sections

Contents

Structure
Preparation
Applications
References
External links

==Structure== DAB is symmetric about the central carbon bond between both ring structures. In the crystal, the rings of each molecule are co-planar and the amine units connect molecules to form an intermolecular 3-dimensional hydrogen bond network.