WAPL
Sign in to saveAlso known as FOE, KIAA0261, WAPAL, WAPL cohesin release factor
Wings apart-like protein homolog (WAPL) is a protein that in humans is encoded by the WAPAL gene. WAPL is a key regulator of the Cohesin complex which mediates sister chromatid cohesion, homologous recombination and DNA looping. Cohesin is formed of SMC3, SMC1, RAD21 and either SA1 or SA2. Cohesin has a ring-like arrangement and it is thought that it associates with the chromosome by entrapping it whether as a loop of DNA, a single strand or a pair of sister chromosomes. WAPL forms a complex with PDS5A or PDS5B and releases cohesin from DNA by opening the interface between SMC3 and RAD21.
Gene data
WAPL- Name
- WAPL cohesin release factor
- Type
- protein-coding
- Aliases
- FOE, KIAA0261, WAPAL
Involved in several processes, including negative regulation of DNA replication; negative regulation of chromatin binding activity; and regulation of sister chromatid cohesion. Located in several cellular components, including Golgi apparatus; intercellular bridge; and microtubule cytoskeleton. [provided by Alliance of Genome Resources, Apr 2022]
via MyGene.info
Gene · Ensembl
WAPL cohesin release factor
- Symbol
- WAPL
- Biotype
- Protein coding
- Organism
- Homo sapiens
- Location
- 10:86,435,256-86,521,843
- Strand
- Reverse (−)
- Assembly
- GRCh38
via Ensembl · EMBL-EBI
Wikidata facts
- Image
- Crystal Structure of Human WAPL (PDB 4K6J).png
Show 5 more facts
- HomoloGene ID
- 41002
- genomic start
- 86435256
- exact match
- identifiers.org/ncbigene/23063
- genomic end
- 86521792
- cytogenetic location
- 10q23.2
Sources (3)
via Wikidata · CC0
~2 min read
Article
4 sectionsContents
- Interphase
- Prophase pathway
- Mechanism
- References
Wings apart-like protein homolog (WAPL) is a protein that in humans is encoded by the WAPAL gene. WAPL is a key regulator of the Cohesin complex which mediates sister chromatid cohesion, homologous recombination and DNA looping. Cohesin is formed of SMC3, SMC1, RAD21 and either SA1 or SA2. Cohesin has a ring-like arrangement and it is thought that it associates with the chromosome by entrapping it whether as a loop of DNA, a single strand or a pair of sister chromosomes. WAPL forms a complex with PDS5A or PDS5B and releases cohesin from DNA by opening the interface between SMC3 and RAD21.
== Interphase == thumb|left|Structure of PDS5B (blue), WAPL (green) and IP6 (PDB 5HDT) (Ouyang et al., 2016) Cohesin loading begins in telophase and is mediated by NIPBL and its binding partner MAU2. In G1, WAPL forms a complex with PDS5 and removes cohesin from the DNA but it is reloaded by NIPBL-MAU2. The equilibrium between loading and release give cohesin a DNA residence time of 20 minutes. During DNA replication, a fraction of cohesin is acetylated and binds to Sororin making it resistant to WAPL and able to hold sister chromatids together. This is crucial for the maintenance of sister chromatid cohesion because once the sister chromatids are separated, cohesion cannot be reestablished.